The 22-year-old actress' "sensuality" and "beauty" saw off stiff competition from Angelina Jolie, Beyoncé Knowles and Pamela Anderson.
The adult magazine writes: "Scarlett Johansson is the apex of beauty and sensuality - from her porcelain skin to her fully feminine figure to her mysterious charisma, which is at once palpable and indefinable."
While the magazine makes it clear that Scarlett is their sexiest woman, the other 24 beauties are listed in no particular order.
Other names to make it onto the sexy list include Jennifer Lopez, Paris Hilton, Carmen Electra and Jessica Alba.
Meanwhile, Scarlett is being tipped to play adult movie superstar Jenna Jameson in a movie adaptation of her autobiography 'How To Make Love Like A Porn Star: A Cautionary Tale'.
Jenna, 32, revealed her admiration for the 'Match Point' actress last month.
She said: "We're looking at Scarlett Johansson. She's my choice. I think she's beautiful."